High Performance Liquid Chromatographie Determination of Ginseng Saponin by Ultraviolet Derivatisation

Abstract
Ginsenosides Rb1, b2, c, d, e and g1 quantitatively reacted with benzoyl chloride in pyridine to afford the respective derivatives having strong UV absorption. The UV–derivatized saponins were separated by high performance liquid chromatography (HPLC), and the saponin–contents of the various Ginseng varieties were determined. It is possible to distinguish white Ginseng from the root hairs of Ginseng from the root hairs of Ginseng by the pattern analysis of HPLC.