Pyruvate decarboxylase is like acetolactate synthase (ILV2) and not like the pyruvate dehydrogenase E1 subunit
- 27 March 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 246 (1-2), 1-5
- https://doi.org/10.1016/0014-5793(89)80241-8
Abstract
Protein sequences of pyruvate decarboxylase (PDC) derived from cloned yeast (Saccharomyces cerevisiae) and bacterial (Zymomonas mobilis) genes were compared with each other and with sequence databases. Extensive sequence similarities were found between them and with two others: cytochrome-linked pyruvate oxidase from Escherichia coli and acetolactate synthase (ilvI in E. coli; ILV2 gene in S. cerevisiae). All catalyse decarboxylation of pyruvate using thiamine pyrophosphate (TPP) as cofactor. General overall similarity suggests common ancestry for these enzymes. None of the sequences was similar to the E1 component of pyruvate dehydrogenase from E. coli which also decarboxylates pyruvate with the help of TPP.Keywords
This publication has 16 references indexed in Scilit:
- “Homology” in proteins and nucleic acids: A terminology muddle and a way out of itCell, 1987
- A sensitive procedure to compare amino acid sequencesJournal of Molecular Biology, 1987
- Nucleotide sequence and deduced amino add sequence ofEscherichia colipyruvate oxidase, a lipid-activated flavoproteinNucleic Acids Research, 1986
- AnalysIs of the primary structure and promoter function of a pyruvate decarbozylase gene (PDCI) fromSaceharomyces cerevisiaeNucleic Acids Research, 1986
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- Molecular structure ofilvIHand its evolutionary relationship toilvGinEscherichia coliK12Nucleic Acids Research, 1983
- [47] Establishing homologies in protein sequencesMethods in enzymology, 1983
- STRUCTURE‐FUNCTION RELATIONSHIPS IN PYRUVATE DECARBOXYLASE OF YEAST AND WHEAT GERM*†Annals of the New York Academy of Sciences, 1982
- Molecular basis of valine resistance in Escherichia coli K-12.Proceedings of the National Academy of Sciences, 1981
- Catalytic Functions of Thiamin DiphosphateAnnual Review of Biochemistry, 1969