Biological properties of human interferon fractions obtained by Blue Sepharose chromatography
- 1 September 1980
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 63 (3-4), 253-261
- https://doi.org/10.1007/bf01315031
Abstract
Human interferons of lymphoid and nonlymphoid origins were segregated into several fractions by virtue of their elution properties from Blue Sepharose. According to desorption of the main portion of antiviral activity, three major classes could be discerned: 1. Leukocyte interferons eluting predominantly in 0.5m NaCl buffer, 2. Lymphoblastoid interferons eluting mostly in 1m NaCl buffer, and 3. Cell culture-derived fibroblast and bladder carcinoma interferons requiring ethylene glycol in addition to 1m NaCl for elution. Desorption behavior from Blue Sepharose did not necessarily correlate with the presence of specific antigenic markers and no consistent segregation of Le and F antigens in individual fractions was observed. All fractions exhibited comparable activity in heterologous sheep and homologous human cells. Therefore, no distinctive biological features could be associated with multiple interferon species isolated by Blue Sepharose chromatography.This publication has 23 references indexed in Scilit:
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