Enantiomer separation using mobile and immobile cyclodextrin derivatives with electromigration

Abstract

The principle of electrochromatography is applied to enantiomer separation with wall‐coated capillaries of 50μm (ID). As chiral stationary phase an immobilized polysiloxane containing chemically bonded monokis‐6‐O‐octamethylene‐permethyl‐cyclodextrin (β‐ or γ‐Chirasil‐Dex) was used. The results are compared with the conventional method of adding cyclodextrin derivatives as chiral pseudostationary phase to the running buffer.