Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase
- 10 November 2000
- journal article
- editorial
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 290 (5494), 1159-1162
- https://doi.org/10.1126/science.290.5494.1159
Abstract
The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families.Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.Keywords
This publication has 28 references indexed in Scilit:
- Genetic Requirements for Inheritance of RNAi in C. elegansScience, 2000
- RNAiCell, 2000
- RNA interference: genetic wand and genetic watchdogNature Cell Biology, 2000
- Posttranscriptional Gene Silencing in Neurospora by a RecQ DNA HelicaseScience, 1999
- A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in PlantsScience, 1999
- The yeast exosome and human PM-Scl are related complexes of 3' right-arrow 5' exonucleasesGenes & Development, 1999
- hnRNP complexes: composition, structure, and functionCurrent Opinion in Cell Biology, 1999
- Do unintended antisense transcripts contribute to sense cosuppression in plants?Trends in Genetics, 1999
- Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas.Plant Cell, 1997
- Structure and inheritance of sense and anti-sense transcripts from a transposon in the green alga Chlamydomonas reinhardtiiJournal of Molecular Biology, 1991