Effect of diphosphonates on the synthesis of prostaglandins in cultured calvaria cells

Abstract

In calvaria cells cultured for 6 days in the presence of dichloromethanediphosphonate (Cl₂MDP) (0.025 – 250 µM), the synthesis of prostaglandin E₂ (PGE₂) was inhibited by up to 90 %. Inhibition of PGE₂ synthesis might be one mechanism whereby this diphosphonate inhibits bone resorption. This effect is not common to all diphosphonates since 25 µM 3-amino-1-hydroxypropane-1,1-diphosphonate (AHPDP) stimulated the synthesis of PGE₂.