Monoclonal IgM in a patient with paraproteinemic polyneuropathy binds to gangliosides containing disialosyl groups

Abstract

Monoclonal IgM K from a patient with polyneuropathy associated with paraproteinemia was found to bind to several polysialogangliosides. Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve gangliosides with the patient's serum followed by radioiodinated goat antihuman IgM. The latter technique showed that the IgM paraprotein reacted with a number of gangliosides. In an ELISA the IgM paraprotein reacted strongly with GD₂, GD₃, GD_1b, and GT_1b, but not with GM₁, GM₃, and GD_1a. Thus, the epitope for the patient's IgM paraprotein appears to involve the disialosyl configurations.