Partial purification and characterization of (Na++K+)-ATPase from garfish olfactory nerve axon plasma membrane
- 1 October 1981
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 63 (1-2), 147-156
- https://doi.org/10.1007/bf01969455
Abstract
The (Na++K+)-ATPase of garfish olfactory nerve axon plasma membrane was purified about sixfold by treatment of the membrane with sodium dodecyl sulfate followed by sucrose density gradient centrifugation. The estimated molecular weights of the two major polypeptide components of the enzyme preparation on sodium dodecyl sulfate gels were 110,000 and 42,000 daltons, which were different from those of the corresponding peptides of rabbit kidney (Na++K+)-ATPase. No carbohydrate was detected in the 42,000-dalton component either by the periodic acid-Schiff reagent or by the more sensitive concanavalin A-peroxidase staining procedure. The molecular properties of the garfish (Na++K+)-ATPase, such as theK m for ATP, pH optimum, energies of activation, Na and K ion dependence and vanadium inhibition, were, however, similar to those of the kidney enzyme. The partially purified garfish (Na++K+)-ATPase was reconstituted into phospholipid vesicles by a freeze-thaw-sonication procedure. The reconstituted enzyme was found to catalyze a time and ATP dependent22Na+ transport. The ratio of22Na+ pumped to ATP hydrolyzed was about 1; under the same reconstitution and assay conditions, eel electroplax (Na++K+)-ATPase, however, gave a22Na+ pumped to ATP hydrolyzed ratio of nearly 3.This publication has 37 references indexed in Scilit:
- Polypeptide components of the (Na+K)-ATPase of garfish olfactory nerve axon plasma membraneLife Sciences, 1979
- Biochemical Mechanism of the Sodium PumpAnnual Review of Physiology, 1979
- Characterization of a new photoaffinity derivative of ouabain: labeling of the large polypeptide and of a proteolipid component of the (sodium-potassium ion)-dependent ATPaseBiochemistry, 1978
- Purification from brain of an intrinsic membrane protein fraction enriched in (Na+ + K+)-ATPaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- A reconstituted Na++K+ pump in liposomes containing purified (Na++K+-ATPase from kidney medullaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- The polypeptide and the phospholipid components of axon plasma membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Coupled Na+K+ transport in vesicles containing a purified (NaK)-ATPase and only phosphatidyl cholineBiochemical and Biophysical Research Communications, 1976
- ISOLATION AND CHARACTERIZATION OF PLASMA MEMBRANE FRACTIONS FROM GARFISH LEPISOSTEUS OSSEUS OLFACTORY NERVEThe Journal of cell biology, 1974
- Composition and characterization of the lipids of garfish (Lepisosteus osseus) olfactory nerve, a tissue rich in axonal membraneBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959