Recovery of DNA from the Acinetobacter calcoaceticus chromosome by gap repair
- 1 October 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 172 (10), 6169-6172
- https://doi.org/10.1128/jb.172.10.6169-6172.1990
Abstract
A strain of Acinetobacter calcoaceticus that demonstrates unusually high competence for natural transformation by linear DNA has proven valuable for analysis of genes and gene clusters associated with aromatic catabolism. The transformation system allowed gap repair to be used to recover mutant chromosomal DNA within recombinant plasmids. The sizes of the recovered fragments, 5 and 7 kilobase pairs in length, indicate that gap repair will be a useful procedure for isolation of wild-type and modified gene clusters from the A. calcoaceticus chromosome.This publication has 24 references indexed in Scilit:
- Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteriaGene, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionMethods in Enzymology, 1987
- Double-stranded gap repair in the photosynthetic prokaryote Synechococcus R2Proceedings of the National Academy of Sciences, 1986
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- The double-strand-break repair model for recombinationCell, 1983
- [14] Genetic applications of yeast transformation with linear and gapped plasmidsMethods in Enzymology, 1983
- A rapid boiling method for the preparation of bacterial plasmidsAnalytical Biochemistry, 1981
- The β-Ketoadipate PathwayPublished by Elsevier ,1973
- Regulation of the Enzymes of the β‐Ketoadipate Pathway in Moraxella calcoaceticaEuropean Journal of Biochemistry, 1968