Antibody Production in Baculovirus-Infected Insect Cells
- 1 July 1990
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 8 (7), 651-654
- https://doi.org/10.1038/nbt0790-651
Abstract
We have employed the baculovirus expression system for the production of a mouse monoclonal IgG antibody directed against lipoprotein I of Pseudomonas aeruginosa. Both light and heavy chain cDNAs were introduced into the baculovirus genome in a single step of homologous recombination. Insect cells that were infected with the recombinant virus stably secreted antigen-binding and glycosylated antibody molecules capable of binding the complement component C1q.Keywords
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