Construction of plasmids carrying the cI gene of bacteriophage lambda.
- 1 November 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (11), 4174-4178
- https://doi.org/10.1073/pnas.73.11.4174
Abstract
By techniques of recombination in vitro, a plasmid bearing the repressor gene (cI) of bacteriophage .lambda. fused to the promoter of the lac operon [Escherichia coli] was constructed. Strains carrying this plasmid overproduce .lambda. repressor. This functional cI gene was reconstituted by joining DNA fragments bearing different parts of that gene. Flush end fusion techniques, involving no sequence overlap, were necessary for the construction; in certain cases, the abutting of the DNA molecules bearing ends generated by different restriction endonucleases creates a sequence at the junction which is recognized by 1 of the restriction endonucleases.This publication has 19 references indexed in Scilit:
- Autoregulation and Function of a Repressor in Bacteriophage LambdaScience, 1976
- Two restriction endonucleases from Bacillus globiggiNucleic Acids Research, 1976
- Amplification and characterization of a β-globin gene synthesized in vitroCell, 1976
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975
- Recognition sequence of restriction endonuclease III from Hemophilus influenzaeJournal of Molecular Biology, 1975
- Control Elements in the DNA of BacteriophageCold Spring Harbor Symposia on Quantitative Biology, 1974
- Structure of the λ OperatorsNature, 1973
- DNA Substrate Site for the EcoRII. Restriction Endonuclease and Modification MethylaseNature New Biology, 1973
- Recognition Sequence of a Restriction EnzymeNature New Biology, 1973
- CXII. Total synthesis of the structural gene for an alanine transfer RNA from yeast. Enzymic joining of the chemically synthesized polydeoxynucleotides to form the DNA duplex representing nucleotide sequence 1 to 20Journal of Molecular Biology, 1972