Regeneration of whole plants from callus culture of diverse genetic lines of Pisum sativum L.

Abstract

Sixteen genetic lines of peas were screened for their ability to regenerate whole plants from callus cultures. Epicotyl sections from germinating seeds were placed on callus-inducing medium; the resulting callus was subcultured monthly and was tested every other month for its regeneration ability. Six lines were found that would regenerate after 2 months' growth as callus. Four of these continued to regenerate after 4 months and, of these, two after 6 months. The cultivars “Frosty” and “Alaska” were among the lines that would not regenerate at all.