Genomic restriction map of the extremely thermophilic bacterium Thermus thermophilus HB8
Open Access
- 1 January 1993
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 175 (1), 103-110
- https://doi.org/10.1128/jb.175.1.103-110.1993
Abstract
A physical map of the chromosome of the extremely thermophilic eubacterium Thermus thermophilus HB8 has been constructed by using pulsed-field gel electrophoresis techniques. A total of 26 cleavage sites for the rarely cutting restriction endonucleases HpaI, MunI, and NdeI were located on the genome. On the basis of the sizes of the restriction fragments generated, the genome size was estimated to be 1.74 Mbp, which is significantly smaller than the chromosomes of Escherichia coli and other mesophiles. Partial digestion experiments revealed the order of the six HpaI bands on the chromosome. Hybridization of isolated restriction fragments to pulsed-field gel-separated restriction digestions confirmed the deduced order of the HpaI fragments and allowed ordering and alignment of the NdeI and MunI fragments. In addition, 16 genes or gene clusters cloned from several different Thermus strains were located on the T. thermophilus HB8 chromosomal map by hybridization of gene probes to pulsed-field gel-resolved restriction digestions.Keywords
This publication has 38 references indexed in Scilit:
- Analysis of the spc ribosomal protein operon of Thermus aquaticusEuropean Journal of Biochemistry, 1991
- A plasmid vector for an extreme thermophile,Thermus thermophilusFEMS Microbiology Letters, 1990
- A plasmid vector for an extreme thermophile, Thermus thermophilusFEMS Microbiology Letters, 1990
- The 23S ribosomal RNA higher‐order structure of Pseudomonas cepacia and other prokaryotesEuropean Journal of Biochemistry, 1989
- Genome comparison of Lactococcus strains by pulsed-field gel electrophoresisFEMS Microbiology Letters, 1989
- Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8European Journal of Biochemistry, 1987
- An unusual rRNA operon constellation: in Thermus thermophilus HB8 the 23S/5S rRNA operon is a separate entity from the 16S rRNA operonBiochimie, 1987
- A Physical Map of the Escherichia coli K12 GenomeScience, 1987
- Plasmid curing in Thermus thermophilus and Thermus flavusFEBS Letters, 1983
- Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a Nonsporulating Thermophilic Bacterium from a Japanese Thermal SpaInternational Journal of Systematic and Evolutionary Microbiology, 1974