Paper Electrophoresis of Carbohydrates in Sulphonated Phenylboronic Acid Buffer.

Abstract

The paper electrophoresis of some carbohydrates in sulphonated phenylboronic acid buffers is described. These allow electrophoresis to be run at neutral pH values and are therefore useful for the separation and characterisation of alkali-labile carbohydrate derivatives. Specific configurations are required for the hydroxyls for complex formation and information of structural significance can be obtained for unknown carbohydrates. The new buffers are a useful complement to those previously described, especially for the separation of glycitols.