Organotypic development of the organ of Corti in culture

Abstract

The preservation and development of the innervation pattern in the organ of Corti have been studied in culture up to 27 daysin vitro. The explants were obtained from the newborn mouse. Segments of the cochlear duct dissected together with the appropriate sectors of the spiral ganglion may retain their structural organization for about two weeks. Maturation of some nonneuronal elements which occurs during that time is followed by a subsequent regression of the organ. Only a fraction of the explanted neurons survive. However, the surviving neurons, if connected with the hair cell region, maintain a complex peripheral innervation pattern that contains all the major fibre components which characterize the normal pattern in a young mouse. The peripheral innervation pattern in culture seems largely composed of preserved fibres, that is, of fibres which at the time of explantation have already ramified within the organ of Corti. Nonetheless, there is evidence for growth or maturation, in culture, of at least some peripheral processes of the spiral neurons. Thus, only in older cultures is the innervation of the apical tip established. Likewise, it is only in older expiants that the inner spiral bundle becomes prominent. Spiral neurons survive in culture in several modes. Most frequently, the central process is altogether absent and the neuron is effectively a unipolar cell which maintains only the peripheral process. A distinct minority of neurons is bipolar possessing both the peripheral process and a central axon which grows freely, though no central target is present. A neuron may survive also as a unipolar or, rarely, as a bipolar cell with no processes entering the organ of Corti. The observations imply that (1) most or all major fibre systems in the organ of Corti carry components of spiral neuron origin; (2) a small population of spiral neurons innervating a short segment of the organ contributes importantly not only to the radial but also to the spiral innervation of the segment.