Effects of Transient Forebrain Ischemia and Pargyline on Extracellular Concentrations of Dopamine, Serotonin, and Their Metabolites in the Rat Striatum as Determined by In Vivo Microdialysis
- 1 March 1990
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 54 (3), 801-808
- https://doi.org/10.1111/j.1471-4159.1990.tb02322.x
Abstract
Striatal microdialysis was performed in rats subjected to 20 min of transient forebrain ischemia produced by occlusion of the carotid arteries during hemorrhagic hypotension. Extracellular changes of dopamine, serotonin, and their metabolites were monitored before, during, and after the ischemic insult at 10-min intervals by on-line HPLC analysis. During ischemia, extracellular dopamine increased dramatically (156 times baseline), as did 3-methoxytyramine (3-MT), whereas 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) decreased (15–25% of baseline). Upon reperfusion, dopamine was cleared from the extracellular fluid within 40 min and reached a stable level (70% of baseline). DOPAC and HVA increased (250–330%) transiently and reached their maximum 1 h following reperfusion, whereas 3-MT decreased to undetectable levels within 20 min. Although baseline levels of serotonin were not detectable, serotonin and 5-hydroxyindoleacetic acid showed a qualitatively similar temporal pattern to dopamine and its acid metabolites. Killing rats by cervical dislocation produced changes in extracellular dopamine, serotonin, and their metabolites that were almost identical to those seen during ischemia. Pargyline pretreatment 2 h before ischemia had marginal effects on the postischemic clearing of dopamine. The pargyline pretreatment, however, did increase the survival rate of rats subjected to ischemia, and this protective effect might be due to the pargyline-induced blockade of the postischemic monoamine oxidase-mediated increase in dopamine metabolism and the concurrent production of the potentially neurotoxic molecule, hydrogen peroxide.Keywords
This publication has 43 references indexed in Scilit:
- Focal ischemia enhances choline output and decreases acetylcholine output from rat cerebral cortex.Stroke, 1989
- Striatal dopamine in acute cerebral ischemia of stroke-resistant rats.Stroke, 1988
- Dopamine depletion protects striatal neurons from ischemia-induced cell deathLife Sciences, 1988
- Scope and limitations of in vivo brain dialysis: A comparison of its application to various neurotransmitter systemsLife Sciences, 1987
- Direct evidence of acute, massive striatal dopamine release in gerbils with unilateral strokes.Stroke, 1987
- Brain anoxia releases striatal dopamine in ratsLife Sciences, 1986
- Nerve terminal damage in cerebral ischemia: protective effect of alpha-methyl-para-tyrosine.Stroke, 1985
- Elevation of the Extracellular Concentrations of Glutamate and Aspartate in Rat Hippocampus During Transient Cerebral Ischemia Monitored by Intracerebral MicrodialysisJournal of Neurochemistry, 1984
- Temporal profile of neuronal damage in a model of transient forebrain ischemiaAnnals of Neurology, 1982
- Extracellular potassium and blood flow in the post-ischemic rat brainPflügers Archiv - European Journal of Physiology, 1980