Simple, Rapid Cleanup Method for Analysis of Aflatoxins and Comparison with Various Methods

Abstract

A method is described for simple and rapid determination of aflatoxins in corn, buckwheat, peanuts, and cheese. Aflatoxins were extracted with chloroform-water and were purified by a Florisil column chromatographic procedure. Column eluates were concentrated and spotted on a high performance thin layer chromatographic (HPTLC) plate, which was then developed in chloroform-acetone (9 + 1) and/or ethermethanol- water (94 + 4.5 + 1.5)orchloroform-isopropanol-acetone (85 + 5 + 10). Each aflatoxin was quantitated by densitometry. The minimum detectable aflatoxin concentrations (μg/kg) in various test materials were 0.2, B₁; 0.1, B₂; 0.2, G₁; 0.1, G₂; and 0.1,M₁.Recoveries of the aflatoxins added to corn, peanut, and cheese samples at 10-30 μg/kg were > 69% (aflatoxin G₂) and averaged 91%, B₁; 89%, B2; 91%, G₁; 78%, G₂; and 92%, M1. The simple method described was compared with the AOAC CB method, AOAC BF method, and AOAC milk and cheese method. These methods were applied to corn, peanut, and cheese composites spiked with known amounts of aflatoxins, and to naturally contaminated buckwheat and cheese. Recoveries were much lower for the BF method compared with our simple method and the CB method.