Concentration of Corticotropin Releasing Factor by Chromatography on Carboxymethylcellulose
- 1 January 1959
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 100 (1), 138-139
- https://doi.org/10.3181/00379727-100-24551
Abstract
Neurohypophysial extracts of commercial origin were chromatographed on carboxymethylcellulose cation exchange adsorbent under conditions of pH and ionic strength gradient in view of separating corticotropin releasing factor (CRF). Evidence for the existence of such a material has been presented previously (Guillemin et al., Endocrinology 60, 488, 1957; Schally et al. Biochem. Jour. 70, 97, 1958). An in vivo assay was used to assess CRF activity. The method described is useful in large scale preparations. Fractions containing CRF were regularly found to emerge before lysine vasopressin and were well separated from oxytocin.Keywords
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