Use of Uncoupling Acridine Dyes as Stoichiometric Energy Probes in Chloroplasts
Open Access
- 1 December 1972
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 50 (6), 713-718
- https://doi.org/10.1104/pp.50.6.713
Abstract
In a suspension of spinach chloroplasts the fluorescence of atebrin and other uncoupling acridine dyes is quenched upon energization which is associated with a proportional binding of the dyes to the organelles. There is a stoichiometric relation between the amount of dye bound and the actual steady state level of energy. When the concentration of atebrin is increased in energized chloroplasts the fluorescence is completely quenched until a certain concentration is attained above which the response sharply declines. Such titrations with atebrin were carried out under conditions of partial electron transport governed by photosystems I and II, in the presence of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea and cyanide, respectively, and of complete electron transport governed by the two photo-systems. The sum of the saturating amounts of atebrin obtained in these partial electron flow systems equals that obtained in the complete system. This lends strong support to the view that two sites of energy conservation are coupled to the linear photosynthetic electron transport.Keywords
This publication has 22 references indexed in Scilit:
- Localization of a site of energy coupling between plastoquinone and cytochrome f in the electron-transport chain of spinach chloroplastsBiochemistry, 1972
- The Membrane Structure Studied with Cationic DyesEuropean Journal of Biochemistry, 1972
- The effect of temperature on energy‐linked functions in chloroplastsFEBS Letters, 1971
- Fluorescent probe of the hydrogen ion concentration in ethylenediaminetetraacetic acid particles of beef heart mitochondriaBiochemistry, 1971
- Energy Transduction in Mitochondrial FragmentsEuropean Journal of Biochemistry, 1971
- On the mechanism of the energy‐dependent quenching of atebrin fluorescence in isolated chloroplastsFEBS Letters, 1971
- Two sites for adenosine triphosphate formation in photosynthetic electron transport mediated by photosystem I. Evidence from digitonin subchloroplast particlesBiochemistry, 1971
- Relationship between light‐induced quenching of atebrin fluorescence and ATP formation in Rhodospirillum Rubrum chromatophoresFEBS Letters, 1971
- Oxidative phosphorylation in Azotobacter vinelandii. Atebrin as a fluorescent probe for the energized stateBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1970
- Quenching of uncoupler fluorescence in relation on the “energized state” in chloroplastsFEBS Letters, 1970