Artificial cell-microencapsulated phenylalanine ammonia-lyase
- 1 February 1984
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 10 (1-3), 57-59
- https://doi.org/10.1007/bf02783735
Abstract
Phenylalanine ammonia-lyase (PAL) is immobilized in collodion artificial cells. Once technical problems associated with the encepsulation of this enzyme were solved, the enzyme kinetics were compared to PAL in free solution. Microencapsulated PAL has an apparent enzyme activity that is 20% of the activity of enzyme in free solution. TheK m for both free and immobilized PAL is 475 μM. TheV m for the microen-capsulated PAL is 9 μM/min, whereas that of PAL in free solution is 55 μM/min.Keywords
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