Protein-binding of small molecules: New gel filtration method
- 1 December 1968
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 20 (Supplement), 150S-156S
- https://doi.org/10.1111/j.2042-7158.1968.tb09877.x
Abstract
The use of frontal analysis chromatography on Sephadex columns, previously described for protein-protein interactions (Nichol & Winzor, 1964) has been extended to the treatment of protein-small molecule interaction. Chromatograms obtained with bovine serum albumin and three different sulphonamides (sulphanilamide, sulphapyridine and sulphamethoxypyridazine) were found to conform to the theoretical patterns, and the values for the fraction of drug bound were in good agreement with those obtained by equilibrium dialysis.Keywords
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