Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits
- 1 June 1995
- journal article
- Published by Springer Nature in Nature
- Vol. 375 (6532), 549-554
- https://doi.org/10.1038/375549a0
Abstract
Recent advances in understanding of bacterial aromatic polyketide biosynthesis allow the development of a set of design rules for the rational manipulation of chain synthesis, reduction of keto groups and early cyclization steps by genetic engineering. The concept of rational design is illustrated by the preparation of Streptomyces strains that produce two new polyketides by expression of combinations of appropriate enzymatic subunits from naturally occurring polyketide synthases. The potential for generating molecular diversity within this class of molecules by genetic engineering is enormous.Keywords
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