Isolation of Specific Antibody to Plant Viruses by Acid Sucrose Density Gradient Centrifugation

Abstract

Antibody preparations specific to southern bean mosaic (cowpea strain) and soybean mosaic viruses were isolated by reaction of virus antigen with immune serum followed by acid dissociation of immune complexes. Dissociated entities were separated by using acid sucrose density gradient centrifugation to prepare immunologically reactive specific antibody. Antibody to soybean mosaic virus was electrophoretically pure and remained reactive after lyophilization.