The influence of the host on expression of intestinal microbial enzyme activities involved in metabolism of foreign compounds

Abstract

The activities of four enzymes (.beta.-glucosidase, .beta.-glucuronidase, nitrate reductase and nitroreductase) in selected intestinal bacteria (Escherichia coli, Clostridium sp., Streptococcus sp., Bacteroides sp. and Lactobacillus salivarius) were measured after growth in vitro and in vivo. The five strains differed in their activities with Clostridium sp. being the most active for .beta.-glucosidase, .beta.-glucuronidase and nitroreductase, and E. coli the most active producer of nitrate reductase. Enzyme activity in vitro tended to be higher than in vitro but there were instances where the comparative activities were reversed.