Pre-Unfolding Resonant Oscillations of Single Green Fluorescent Protein Molecules
- 12 August 2005
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 309 (5737), 1096-1100
- https://doi.org/10.1126/science.1115001
Abstract
Fluorescence spectroscopy of a green fluorescent protein mutant at single-molecule resolution has revealed a remarkable oscillatory behavior that can also be driven by applied fields. We show that immediately before unfolding, several periodic oscillations among the chemical substates of the protein chromophore occur. We also show that applied alternating electric or acoustic fields, when tuned to the protein characteristic frequencies, give rise to strong resonance effects.Keywords
This publication has 40 references indexed in Scilit:
- Unfolding of Green Fluorescent Protein mut2 in wet nanoporous silica gelsProtein Science, 2005
- Kinetics of Acid-Induced Spectral Changes in the GFPmut2 ChromophoreJournal of the American Chemical Society, 2004
- Single molecule spectroscopic characterization of GFP‐mut2 mutant for two‐photon microscopy applicationsMicroscopy Research and Technique, 2004
- Confocal laser scanning microscopy to study formation and properties of polyelectrolyte nanocapsules derived from CdCO3 templatesMicroscopy Research and Technique, 2002
- Control, exploitation and tolerance of intracellular noiseNature, 2002
- Dynamics of green fluorescent protein mutant2 in solution, on spin‐coated glasses, and encapsulated in wet silica gelsProtein Science, 2002
- Crowding and hydration effects on protein conformation: a study with sol-gel encapsulated proteins 1 1Edited by P. E. WrightJournal of Molecular Biology, 2001
- THE GREEN FLUORESCENT PROTEINAnnual Review of Biochemistry, 1998
- FACS-optimized mutants of the green fluorescent protein (GFP)Gene, 1996
- Vibrational normal-mode spectrum of globular proteinsPhysical Review B, 1993