The Effect of Hypophysectomy and Growth Hormone Administration on Pre-Prosomatostatin Messenger Ribonucleic Acid in the Periventricular Nucleus of the Rat Hypothalamus*
- 1 February 1988
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 122 (2), 586-591
- https://doi.org/10.1210/endo-122-2-586
Abstract
Physiological evidence suggests that hypothalamic somatostatin (SS) inhibits pituitary GH release and that GH acts through a short-loop feedback mechanism to stimulate SS secretion. The feedback action of GH could be mediated by an effect on SS synthesis, secretion, or both. We hypothesized that GH acts to regulate the expression of the SS gene and that changes in the level of circulating GH would result in corresponding changes in SS mRNA in cells of the periventricular nucleus (PeN) of the hypothalamus. To test this hypothesis we measured the effect of hypophysectomy (HPX) and HPX with bovine GH (bGH) replacement on SS mRNA signal levels in cells of the PeN of the rat brain. We report that HPX male rats treated with bGH have significantly higher PeN SS mRNA signal than their vehicle-treated controls (P < 0.05) and that bGH administration to sham-HPX rats results in elevated PeN SS mRNA signal levels compared to those in sham-HPX rats treated with vehicle (P < 0.05). These observations suggest that GH participates in the regulation of its own secretion by influencing the expression of the SS gene and that one mechanism of short-loop pituitary feedback may involve the modulation of neuropeptide gene expression.This publication has 14 references indexed in Scilit:
- Characterization of Insulin-Like Growth Factor Receptors in Rat Anterior Pituitary, Hypothalamus, and Brain*Endocrinology, 1984
- INTRAVENTRICULARLY INJECTED GROWTH HORMONE STIMULATES SOMATOSTATIN RELEASE INTO RAT HYPOPHYSIAL PORTAL BLOODEndocrinology, 1981
- Effects of Growth Hormone Excess and Deficiency on Hypothalamic Somatostatin Content and Release and on Tissue Somatostatin Distribution*Endocrinology, 1981
- The Effect of Hypophysectomy on Somatostatin-Like Immunoreactivity in Discrete Hypothalamic and Extrahypothalamic Nuclei*Endocrinology, 1980
- Biochemical mapping of somatostatinergic systems in rat brain: Effects of periventricular hypothalamic and medial basal amygdaloid lesions on somatostatin-like immunoreactivity in discrete brain nucleiBrain Research, 1980
- Growth hormone stimulates hypothalamic somatostatinLife Sciences, 1979
- Effect of Hypophysectomy and Growth Hormone Administration on Somatostatin Content in the Rat HypothalamusNeuroendocrinology, 1979
- TISSUE GROWTH HORMONE RELEASE INHIBITING HORMONE-LIKE IMMUNOREACTIVITY IN EXPERIMENTAL HYPOTHYROIDISM AND HYPOPITUITARISMClinical Endocrinology, 1978
- Hypothalamic Somatostatin and LH-RH after Hypophysectomy, in Hyper- or Hypo-Thyroidism, and during Anesthesia in RatsExperimental Biology and Medicine, 1978
- Effect of hypophysectomy on hypothalamic somatostatin content in rats.Endocrinologia Japonica, 1976