Identification of two new members of the tubulin family
- 1 January 1995
- journal article
- review article
- Published by Wiley in Cell Motility
- Vol. 31 (4), 255-258
- https://doi.org/10.1002/cm.970310402
Abstract
Analysis of the delta- and epsilon-tubulin sequences indicates that they both consist of two structural domains of which the N-terminal domain can bind to alpha/beta heterodimers while the C-terminal domain probably binds to a non-tubulin protein. Both additional tubulins probably bind GTP but lack GTPase activity, while their synthesis requires the TCP1 chaperonine but is not autoregulated. Although these properties resemble those of gamma-tubulin, the low sequence identity (Table I) demonstrates that the gamma-, delta-, and epsilon-proteins should be classed as different members of the tubulin family. The identification of these additional members is unexpected. Examination of the cellular expression and distribution of the delta- and epsilon-tubulins, and whether other organisms contain homologous genes, may reveal further features of the eukaryotic cytoskeleton.Keywords
This publication has 8 references indexed in Scilit:
- Analysis of the γ-tubulin sequences: Implications for the functional properties of γ-tubulinJournal of Cell Science, 1995
- β‐Tubulin mutation suppresses microtubule dynamics in vitro and slows mitosis in vivoCell Motility, 1995
- Functional role of a consensus peptide which is common to α‐, β‐, and γ‐tubulin, to actin and centractin, to phytochrome A, and to the TCP1α chaperonin proteinFEBS Letters, 1994
- Microtubule Dynamics Modulated by Guanosine Triphosphate Hydrolysis Activity of β-TubulinScience, 1994
- Identification of peptides within the base binding domains of the GTP- and ATP-specific binding sites of tubulin.Journal of Biological Chemistry, 1994
- Exchangeable GTP binding site of beta-tubulin. Identification of cysteine 12 as the major site of cross-linking by direct photoaffinity labeling.Journal of Biological Chemistry, 1993
- Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulansNature, 1989
- Autoregulated changes in stability of polyribosome-bound beta-tubulin mRNAs are specified by the first 13 translated nucleotides.Molecular and Cellular Biology, 1988