A ribosome‐associated inhibitor of in vitro nonsense suppression in [psi−] strains of yeast
- 10 December 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 225 (1-2), 205-208
- https://doi.org/10.1016/0014-5793(87)81158-4
Abstract
All classes of tRNA-mediated nonsense suppression are much more efficient in yeast cell-free lysates prepared from a [psi +] strain than in those prepared from an isogenic [psi −] strain. Mixed [psi +]/[psi −] lysates do not support efficient suppression. Fractionation of the [psi −] lysate demonstrated the presence of an inhibitor of in vitro suppression that is loosely associated with the 80 S ribosome. The data indicate that the inhibitor is a factor involved in the termination of translation in this simple eukaryote.Keywords
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