Prophage ψ80 is induced in Escherichia coli K12 recA430
- 1 March 1983
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 189 (2), 199-206
- https://doi.org/10.1007/bf00337804
Abstract
Summary In contrast to prophage λ, wild-type prophage ψ80 was induced by UV-irradiation or thymine deprivation in recA430 mutants of E. coli K12. There was no induction of prophage ψ80 in two recombination-deficient mutants recA13 and recA99. Phage ψ80ind3, a non-inducible derivative in a rec + was not induced in a recA430 lysogen. Two other lambdoid prophages were tested for UV-induction in recA430 lysogens: in common with λ prophage, 434 was not induced whereas prophage 21 was induced in 1% of the cells. Induction of RecA430 protein synthesis was 30% of that observed in recA + bacteria at 30 min of post-irradiation incubation, indicating that LexA repressor had been cleaved by RecA430 protease. In lexA1 recA430 and lexA1 recA + bacteria, RecA protein synthesis was not amplified, yet, prophage ψ80 was fully induced. If ψ80cI repressor is inactivated by cleavage by RecA430 protease as is LexA repressor, RecA430 protease can inactivate all the molecules of ψ80cI repressor, its basal level being high enough in a recA430 lysogen. In such a lysogen, a fraction only of 21cI and LexA repressors are cleaved but no molecules of either λcI or 434cI repressor. We postulate that RecA430 protein has an altered pattern of recognition of repressor molecules and a cleavage efficiency which is more efficient the more remote is the repressor conformation from that of λ repressor.Keywords
This publication has 61 references indexed in Scilit:
- The SOS regulatory system of Escherichia coliCell, 1982
- An inducible DNA replication–cell division coupling mechanism in E. coliNature, 1981
- Induction of prophage λ does not require full induction of RecA protein synthesisBiochimie, 1980
- Cleavage of λ repressor and induction of RecA protein synthesis elicited by aflatoxin B1 metabolites in Escherichia coliCarcinogenesis: Integrative Cancer Research, 1980
- Kinetics of recA protein-directed inactivation of repressors of phage λ and phage P22Journal of Molecular Biology, 1980
- E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotideNature, 1980
- Inactivation of prophage λ repressor in VivoJournal of Molecular Biology, 1979
- Purification and Properties of Phage P22 c2 RepressorEuropean Journal of Biochemistry, 1978
- Prophage induction and cell division in E. coliMolecular Genetics and Genomics, 1977
- Repair mechanisms involved in prophage reactivation and UV reactivation of UV-irradiated phage λMutation Research, 1973