Comparison of Candida-Delayed Hypersensitivity Skin Test Size with Lymphocyte Transformation, Migration Inhibitory Factor Production and Antibody Titer

Abstract

There is conflicting evidence concerning the correlation of in vitro lymphocyte transformation (LT) with migration inhibitory factor (MIF) production, in vivo delayed hypersensitivity and antibody formation. In the present study, both normal controls and patients with mucocutaneous candidiasis were assessed for delayed (cell-mediated) hypersensitivity to Candida antigen by skin testing, antigen-induced LT and MIF production. Specific anti-Candida-precipitating antibody levels were determined by counterimmunoelectrophoresis. A significant correlation (p < 0.001) was observed between the size of the skin reaction and the level of Candida in vitro lymphocyte stimulation. Although there was positive qualitative correlation between both delayed skin reaction size and LT compared to MIF production, no significant quantitative correlation with MIF production was observed.