The Isolation of Nuclei and Oxidative Particles from Viable Wheat Embryos.
Open Access
- 1 March 1957
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 32 (2), 124-129
- https://doi.org/10.1104/pp.32.2.124
Abstract
Viable embryos of wheat were prepared in gram quantities and from such preparations nuclei and mitochondria were isolated. Embryos were sectioned on a freezing microtome and then gently homogenized in 2 [image] sucrose. Fractionation was accomplished by differential centrifugation. Nuclei thus isolated were morphologically intact and free from impurities: the cytochrome oxidase of the mitochondria had the highest specific activity yet recorded for plant particles.This publication has 12 references indexed in Scilit:
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