Characterisation of an intermediate in neurophysin biosynthesis in the guinea pig
- 14 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 163 (2), 324-328
- https://doi.org/10.1016/0014-5793(83)80845-x
Abstract
In addition to oxytocin (OT), vasopressin (AVP) and their respective neurophysins (NPs), another [35S]cysteine incorporating component is present in the guinea pig neurohypophysis. Gel filtration and Con A affinity chromatography revealed that this component was larger than NP and was glycosylated. NP-immunoreactivity was assessed using antisera which distinguish the OT- and AVP-related NPs. Whilst the anti-NP antiserum detected only one component (guinea pig NP), the anti-NP antiserum detected both NP and the glycosylated 35S-labelled component. These results suggest that a significant amount of NP in guinea pig neural lobes bears a glycopeptide extension and represents a partially processed form of the AVP precursor in this species.Keywords
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