Barley Malt alpha-Glucosidase. VI. Localization and Development during Barley Germination.

Abstract

It was found that in ungerminated barloy the [alpha]-glucosidase activity was located in the embryo and the aleurone layer. The [alpha]-glucosidase activity was somewhat lower in the aleurone layer than in the embryo. In barley germinated for 48 hours the [alpha]-glucosidase activity was greater than in ungerminated barley but located at the same sites. Especially the activity in the scutellum and the scutellar epithelium was much greater as revealed by the deeper blue stain at these sites. The localization of [alpha]-glucosidase activity in distinct areas of the germinated barley grains shows that the [alpha]-glucosidase activity in barley malt does not originate from microorganisms developed during the germination of the barley.