A new flow chamber and processing electronics for combined laser and mercury arc illumination in an impulscytophotometer flow cytometer

Abstract

A flow cytometer of the impulscytophotometer (ICP)‐type has been developed for two‐parameter flow cytometry. It uses two different methods of excitation simultaneously: an argon laser beam focused onto the cell stream within a modified flow chamber, and the light of a high pressure mercury (Hg) lamp using Köhler illumination. The two fluorescence signals are collected sequentially by the same photomultiplier tube. The signals are processed on a cell‐by‐cell basis using new hardwired analog signal processing electronics enabling their accumulation and display as two‐parameter frequency distribution histograms.