Lipids of Sarcina lutea III. Composition of the Complex Lipids

Abstract

The complex lipids from a strain of S. lutea were isolated and separated into fractions on diethyl-aminoethyl-cellulose-acetate and silicic-acid columns. These fractions were monitored in several thin-layer chromatography systems. The various iipid types were characterized by their behavior in thin-layer systems and by an analysis of their hydrolysis products. The fatty-acid composition of the column fractions was determined by gas-liquid chromatography. A number of components (13) were separated by thin-layer chromatography and characterized. The major components were polyglycerol-phosphatide (17.0%), lipoamino-acids (15.1%), phosphatidyl-glycerol (13.8%), and an incompletely characterized substance (15.0%). Minor constituents included phosphatidyl-inositol (5.5%), phosphatidic-acid (4.2%), phosphatidyl-serine (2.0%) and phosphatidyl-choline (1.0%). No phosphatidyl-ethanolamine was observed.