Identification and Quantitative Analysis of Indole-3-Acetyl-l-Aspartate from Seeds of Glycine max L.
- 1 September 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 70 (3), 749-753
- https://doi.org/10.1104/pp.70.3.749
Abstract
Indole-3-acetyl-L-aspartate (IAAsp) was isolated from seeds of G. max. L. cv. Hark and its identity established by its chromatographic performance and its mass spectral fragmentation. Following acid hydrolysis, the aspartate moiety was the L-enantiomer by reverse phase high performance liquid chromatographic retentiom time of the bisethyl ester derivatized with 2,3,4,6-tetra-O-acetyl-.beta.-D-glycopyranosyl isothiocyanate. Isotope dilution analysis using [14C]IAAsp as internal standard showed that soybean seed contained 10 .mu.mol/kg IAAsp and this accounted for 50% of the total IAA of the seed.Keywords
This publication has 13 references indexed in Scilit:
- Double-standard isotope dilution assayAnalytical Biochemistry, 1981
- The van URK-Salkowski reagent — a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivativesJournal of Chromatography A, 1977
- Metabolism of Indole-3-acetic AcidPlant Physiology, 1977
- A gas-liquid chromatographic-isotope dilution analysis of cysteine, histidine, and tryptophan in acid-hydrolyzed proteinAnalytical Biochemistry, 1976
- Quantitative gas-liquid chromatography and mass spectrometry of the N(O)-perfluorobutyryl-O-isoamyl derivatives of amino acidsAnalytical Biochemistry, 1975
- Metabolism of 2,4-dichlorophenoxyacetic acid. V. Identification of metabolites in soybean callus tissue culturesJournal of Agricultural and Food Chemistry, 1973
- The Relative Amounts and Identification of Some 2,4-Dichlorophenoxyacetic Acid Metabolites Isolated from Soybean Cotyledon Callus CulturesPlant Physiology, 1972
- Growth Substances From Veratrum tenuipetalumPlant Physiology, 1968
- Studies on 3-Indoleacetic Acid Metabolism. II. Some Products of the Metabolism of Exogenous Indoleacetic Acid in Plant TissuesPlant Physiology, 1956
- The Formation of Indoleacetylaspartic Acid in Pea Seedlings.Plant Physiology, 1955