Preparation of yeast mannan derivatives by stearoylation and phosphorylation.

Abstract

The mannan from baker''s yeast was modified by stearoylation and phosphorylation, and the water-soluble part of the resultant product, stearoyl mannan phosphate (SMP), was resolved by gel-filtration with Sephadex G-75 into 2 fractions, SMP-Fr. I and -Fr. II. The former fraction was found to contain 3 components, fatty acid, carbohydrate and phosphate. The SMP-Fr. I was then fractionated by DEAE-Sephadex chromatography to give 4 subfractions, SMP-Fr. I -a, -b, -c, and -d. Each fraction except Fr. I-a was found to contain fatty acid, carbohydrate and phosphate, and to be homogeneous in ultracentrifugal analyses.