A Short Column Procedure for Separating Cytoplasmic Components from Normal and Tumor Tissue

Abstract

A technique for obtaining cell and nuclei-free tissue extracts containing sub-cellular particulates by a method other than centrifugation is described. It consists essentially of grinding the tissue with diatomaceous silica (Celite) and pouring the resulting slurry of silica, ground tissue, and solvent on a short, broad column of the same material and washing or developing with an equal volume of the solvent employed in the extraction grinding. The recovered percolate consists of soluble tissue components, mitochondria, and sub-visible particulates. This filtrate compares favorably with extracts of the same tissue prepd. by centrifugation, and is suitable for certain enzyme studies or for further purification by chromatography or differential centrifugation.