The scanned nanopipette: a new tool for high resolution bioimaging and controlled deposition of biomolecules
- 5 July 2005
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Physical Chemistry Chemical Physics
- Vol. 7 (15), 2859-2866
- https://doi.org/10.1039/b506743j
Abstract
The boundary between the physical and biological sciences has been eroded in recent years with new physical methods applied to biology and biological molecules being used for new physical purposes. We have pioneered the application of a form of scanning probe microscopy based on a scanned nanopipette, originally developed by Hansma and co-workers, for reliable non-contact imaging over the surface of a live cell. We have found that the nanopipette can also be used for controlled local voltage-driven application of reagents or biomolecules and this can be used for controlled deposition and the local delivery of probes for mapping of specific species. In this article we review this progress, focussing on the physical principles and new phenomena that we have observed, and then outline the future applications that are now possible.Keywords
This publication has 54 references indexed in Scilit:
- Nanowire Probes for High Resolution Combined Scanning Electrochemical Microscopy − Atomic Force MicroscopyNano Letters, 2005
- Individual Molecules of Dye‐Labeled DNA Act as a Reversible Two‐Color Switch upon Application of an Electric FieldAngewandte Chemie International Edition, 2004
- An Addressable Antibody Nanoarray Produced on a Nanostructured SurfaceJournal of the American Chemical Society, 2004
- The use of scanning ion conductance microscopy to image A6 cellsMolecular and Cellular Endocrinology, 2004
- Multicomponent Submicron Features of Biomolecules Created by Voltage Controlled Deposition from a NanopipetJournal of the American Chemical Society, 2003
- Dexamethasone and ursodeoxycholic acid protect against the arrhythmogenic effect of taurocholate in an in vitro study of rat cardiomyocytesBJOG: An International Journal of Obstetrics and Gynaecology, 2003
- Protein Nanoarrays Generated By Dip-Pen NanolithographyScience, 2002
- Specialized scanning ion‐conductance microscope for imaging of living cellsJournal of Microscopy, 1997
- Disruption of Microtubules in Cultured Neonatal Rat Cardiomyocytes During Rapid Contractions: Protective effects of beta-adrenoceptor antagonist.Japanese Circulation Journal, 1992
- Single-channel currents recorded from membrane of denervated frog muscle fibresNature, 1976