Fractionation of Transfer Ribonucleic Acids from Torulopsis utilis*
- 1 August 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 62 (2), 161-169
- https://doi.org/10.1093/oxfordjournals.jbchem.a128644
Abstract
The alanine, valine and isoleucine tRNA's were highly purified by chromatography on a DEAE-Sephadex column with the ammonium sul-fate-DMFA, phosphate-DMFA-KCI and borate-KCI systems. The purity of the alanine tRNA I was 64–66%, of the valine tRNA I, 85% and of the isoleucine tRNA, 80%, on the basis of acceptor activity. The alanine and valine tRNA's were resolved into two components. The valine tRNA II was concentrated 11-fold over the original (the purity, 48%) and freed from the valine tRNA I. The final preparations of the alanine, valine and isoleucine tRNA's were digested with pancreatic RNase I [EC 2.7.7.16] and fractionated on a DEAE-cellulose column in 7 M uera according to the chain length. The distribution patterns of oligonucleotide fragments thus obtained were quite characteristic of the specific tRNA's, which in turn supported that the final tRNA preparations were highly purified.Keywords
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