Occurrence of mRNA for Storage Protein in Dry Soybean Seeds1
- 1 November 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 84 (5), 1103-1111
- https://doi.org/10.1093/oxfordjournals.jbchem.a132224
Abstract
Poly(A)-containing RNA has been isolated from the cotyledons of soybean seeds by adsorption on a poly(U)-Sepharose column. Approximately 0.15% of the total soybean RNA applied bound to the column. The bound RNA (poly(A)-containing RNA) was shown to be mRNA by its ability to serve as template in a cell-free system derived from wheat germ. Poly(A)-containing RNA was polydisperse, migrating from approximately 50,000 to 700,000 daltons with a mean of 150,000 daltons in polyacrylamide gel electrophoresis. The size of the poly(A) portion of this RNA was in the range of 55 to 290 nucleotides. The adenylic acid content of the presumed poly(A) fragment was about 95%. The radioactive products of translation directed by the poly(A)-containing RNA in the wheat germ cell-free system were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by immunoprecipitation using antisera against beta-conglycinin and glycinin. The results of this investigation show that mRNAs for the subunit proteins of the major components of a soybean storage protein exist in the poly(A)-containing RNA preparation obtained from the cotyledons of dry soybean seeds.This publication has 14 references indexed in Scilit:
- In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences, 1977
- Beta-conglycinin from soybean proteins. Isolation and immunological and physicochemical properties of the monomeric formsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977
- Translation of poly(A)-containing and poly(A)-free messenger RNA for phenylalanine ammonia-lyase, a plant-specific protein, in a reticulocyte lysateBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Isolation and in vitro translation of zein messenger ribonucleic acidBiochemistry, 1976
- Stabilization of mRNA templates in bovine lens epithelial cellsJournal of Molecular Biology, 1967
- The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresisBiochemical Journal, 1967
- ENZYME ACTIVITIES ASSOCIATED WITH RIBOSOMES FROM SOYBEAN SEEDLINGSPlant and Cell Physiology, 1966
- Ribonucleic acid synthesis in germinating cotton seedsJournal of Molecular Biology, 1966
- BASE COMPOSITION OF FRACTIONATED TRANSFER RIBONUCLEIC ACIDS FROM YEAST1964
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951