GUINEA PIG LYMPHOTOXIN AND COMPARISON OF VARIOUS ASSAY METHODS FOR CYTOTOXICITY

Abstract

SUMMARY The conditions for the production of lymphotoxin from guinea pig lymph node cells stimulated with Phaseolus vulgaris phytohemagglutinin (PHA) are described. A comparison of various assay methods of lymphotoxin, including the incorporation of isotopically labeled compounds, standard cell counting, and the release of isotope from ⁵¹Cr-labeled cells, was made. The method utilizing ¹⁴C-amino acid or ³H-inositol uptake was the most satisfactory in both sensitivity and accuracy. The advantages and disadvantages of other assay methods are also described.