The Relation Between Glucose Repression of Endo-Polygalacturonate Trans-Eliminase and Adenosine 3'5'-Cyclic Monophosphate Levels in Erwinia carotovora

Abstract

The level of intracellular endo-polygalacturonate trans-eliminase (PGTE) activity by E. carotovora on minimal salts medium was affected by the available C source. When cells growing on sodium polypectate were supplemented with glucose, the level of intracellular PGTE activity decreased. Exogenously supplied cyclic (c)AMP reversed repression of intracellular PGTE activity if the substrate of the enzyme, sodium polypectate, was present. Various analogs of cAMP and other nucleotide derivatives failed to reverse glucose repression of PGTE activity. Measurements of cAMP demonstrated that a decrease in cAMP could be correlated with glucose repression of PGTE activity. As the PGTE activity increased in induced cultures, the specific concentration of cAMP increased.