STUDIES ON THE PERMEABILITY OF LIVING CELLS

Abstract

The penetration of 2,-6,-dibromo phenol indophenol into the sap of Valonia follows the course of a bimolecular reaction curve, and the amount of dye in the sap at equilibrium is proportional to the amount of undissociated dye in the external solution. At higher temperatures and lower concentrations the curves are similar in shape to those for 2 consecutive unimolecular reactions. The dye is found in the sap only in a reduced form. Methylene blue, however, is found only in an oxidized form in the sap. The amount is constant at all external and internal changes in pH from 5 to 9. At these pH values methylene blue is completely dissociated. Further indigo dyes are discussed. The electrode potential of the protoplasm of Valonia was found tentatively. The rH of the sap was found to be between 16 and 18.