In vitro Metabolism of DL-AIanine-2-C-14 and Glycine-2-G-14 by Trichinella spiralis Larvae.

Abstract

Trichinella muscle larvae were isolated by pepsin digest and cultured in vitro in a serum-Krebs-Ringer medium or Krebs-Ringer medium containing either dl-alanine-2-C-14 or glycine-2-C-14. Carbon-14 analysis of the larvae revealed a progressive uptake of the C-14-labeled amino acids in both types of media through 48 hours of incubation. Of the total C-14 incorporated by larvae cultured 48 hours in glycine-2-C-14 labeled media about 70 and 84% was precipitable by tungstic acid, and was presumably chiefly in the proteins. Larvae cultured in the dl-alanine-2-C-14-labeled media incorporated about 32-57% of their total C-14 content into material precipitable by tungstic acid.