Isolation and preliminary characterization of bleomycin-resistant mutants from Chinese hamster ovary cells
- 1 April 1981
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 107 (1), 147-153
- https://doi.org/10.1002/jcp.1041070116
Abstract
Stable mutants resistant to an anticancer antibiotic, bleomycin-A2, were selected in Chinese hamster ovary (CHO) cell either spontaneously or after ethylmethane sulfonate mutagenesis. Fluctuation analysis showed that bleomycin resistance occurs in CHO at a rate of 6.50–6.58 × 10−7 mutations per cell per generation. Bleomycin-A2-resistant cell lines exhibited increased resistance to bleomycin analogs—bleomycin-A5, -B2, -B4, and pepleomycin. Colchicine, mitomycin C, and ultraviolet light irradiation inhibited colony formation equally in CHO cells and in bleomycin-resistant mutants. Cell-cell hybridization tests showed that bleomycin-resistance behaves as a dominant trait. Bleomycin-in-activating activity in the mutant cell extracts was three to fourfold higher than that in extracts of the parental CHO cell.Keywords
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