Neuropeptide Y modulates neurotransmitter release and Ca2+ currents in rat sensory neurons
Open Access
- 1 July 1988
- journal article
- research article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 8 (7), 2438-2446
- https://doi.org/10.1523/jneurosci.08-07-02438.1988
Abstract
Using 125I-labeled neuropeptide Y (NPY) and peptide YY (PYY), we demonstrated the existence of specific receptors for these peptides on rat dorsal root ganglion (DRG) cells grown in primary culture. Scatchard analysis of membrane homogenates indicated that the peptides bound to 2 populations of sites, with approximate affinities of 0.08 and 6.5 nM. Only low levels of binding were detected on sympathetic neurons cultured from the same animals or on a variety of neuronal clonal cell lines. the binding of 125I-NPY and 125I-PYY to DRG cell membranes was considerably reduced by the nonhydrolyzable analog of GTP, Gpp(NH)p. The major effect of Gpp(NH)p was to reduce the number of lower-affinity NPY binding sites without altering the number of high-affinity binding sites. NPY potently inhibited Ca2+ currents recorded under voltage clamp in rat DRG cells. Both the transient and sustained portions of the Ca2+ current were inhibited. The inhibitory effects of NPY were completely blocked following treatment of the cells with pertussis toxin. Depolarization elicited a large influx of Ca2+ into DRG neurons as assessed using fura-2-based microspectrofluorimetry. This influx of Ca2+ could be partially inhibited by NPY. Furthermore, NPY effectively inhibited the depolarization-induced release of substance P from DRG cells in vitro. Thus, NPY may be an important regulator of sensory neuron function in vivo.This publication has 40 references indexed in Scilit:
- VIP and PHI coexist with an NPY-like peptide in intramural neurones of the small intestineRegulatory Peptides, 1984
- Differential co-existence of neuropeptide Y (NPY)-like immunoreactivity with catecholamines in the central nervous system of the ratNeuroscience, 1984
- Neuropeptide Y Distribution in the Rat BrainScience, 1983
- Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to α‐adrenoceptor blockadeActa Physiologica Scandinavica, 1982
- Neuropeptide Y: complete amino acid sequence of the brain peptide.Proceedings of the National Academy of Sciences, 1982
- Isolation and characterization of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion.Proceedings of the National Academy of Sciences, 1982
- Neurotransmitters decrease the calcium conductance activated by depolarization of embryonic chick sensory neurones.The Journal of Physiology, 1981
- Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patchesPflügers Archiv - European Journal of Physiology, 1981
- Avian pancreatic polypeptide (APP) immunoreactive neurons in the spinal cord and spinal trigeminal nucleusPeptides, 1981
- Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration.Proceedings of the National Academy of Sciences of the United States of America, 1979