Über das aminoacylierungsverhalten chemisch modifizierter phenylalaninspezifischer transfer‐ribonucleinsäure aus hefe: (1) Glykolspaltung und reduktion zum diol an der 3′‐terminalen ribose
- 1 December 1968
- journal article
- Published by Wiley in FEBS Letters
- Vol. 2 (2), 136-139
- https://doi.org/10.1016/0014-5793(68)80124-3
Abstract
The terminal cis-glycol-group of phenylalanine specific tRNA (tRNAPhe, I) from yeast was oxidized to the dialdehyde by NaIO4 (tRNAPheoxi, II) and subsequently reduced to the diol by NaBH4 (tRNAPheoxi-red, III). In charging experiments it could be shown that tRNAPheoxi-red is still completely specific for phenylalanine. The Michaelis constant of tRNAPheoxi-red remains unchanged when compared with tRNAPhe, while the maximun velocity of charging υmax of tRNAPheoxi-red is decreased to about one half.Keywords
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