Integrated Microfluidic System Enabling (Bio)chemical Reactions with On-Line MALDI-TOF Mass Spectrometry
- 9 July 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 74 (16), 3972-3976
- https://doi.org/10.1021/ac020185n
Abstract
A continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the micro-fluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.Keywords
This publication has 18 references indexed in Scilit:
- Recent developments in detection methods for microfabricated analytical devicesLab on a Chip, 2001
- High quality mechanical etching of brittle materials by powder blastingSensors and Actuators A: Physical, 2000
- Chemical and biochemical microreactorsTrAC Trends in Analytical Chemistry, 2000
- Deep wet etching of borosilicate glass using an anodically bonded silicon substrate as maskJournal of Micromechanics and Microengineering, 1998
- Hydrodynamic Focusing on a Silicon Chip: Mixing Nanoliters in MicrosecondsPhysical Review Letters, 1998
- DNA electrochemical biosensors for environmental monitoring. A reviewAnalytica Chimica Acta, 1997
- Clinical potential of microchip capillary electrophoresis systemsElectrophoresis, 1997
- Metastable fragment-ion analysis in a reflectron instrument with a gridless ion mirrorEuropean Journal of Mass Spectrometry, 1996
- C-Terminal Ladder Sequencing via Matrix-Assisted Laser Desorption Mass Spectrometry Coupled with Carboxypeptidase Y Time-Dependent and Concentration-Dependent DigestionsAnalytical Chemistry, 1995
- Delayed extraction matrix‐assisted laser desorption time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 1995