Frequencies of phosphorylcholine‐specific and T15‐associated 10/13 idiotope‐positive B cells within lipopolysaccharide‐reactive B cells of adult BALB/c mice

Abstract

The frequencies of lipopolysaccharide-reactive B lymphocytes in adult BALB/c spleen cells were determined by limiting dilution experiments to be between 1 in 50 and 1 in 100. Within this reactive population, the frequencies of B lymphocytes specific for phosphorylcholine (PC) and/or binding a TEPC 15-associated idiotope-specific monoclonal antibody (10/13) were found to be between 1 in 50 and 1 in 500. Binding of these antibodies to immobilized PC or to monoclonal idiotope-specific antibodies could be inhibited by soluble PC in a solid-phase radioimmunoassay. Around half the PC-specific B cell clones were found to also bind 10/13 idiotope-specific monoclonal antibody, while the rest were either PC specific, but idiotope-negative or idiotope-positive, and unable to bind immobilized PC.